ELISA

ELISA
(= enzyme-linked immuno-sorbent assay)
A very sensitive technique for the detection of small amounts of protein or other antigenic substance. The basis of the method is the binding of the antigen by an antibody that is linked to the surface of a plate. Formation of an immune complex is detected by use of peroxidase coupled to antibody, the peroxidase being used to generate an amplifying colour reaction. Various ways of carrying out the assay are possible: if the aim is to detect antibody production from a myeloma clone, for example, then the antigen may be bound to the plate, and the formation of the antibody/antigen complex may be detected using peroxidase coupled to an anti-Ig antibody.

Dictionary of molecular biology. 2004.

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